The Central Board of Secondary Education (CBSE) conducts the examination for higher secondary classes each year. Students who are currently studying in their class 12 must be well-aware of the complete exam pattern and syllabus to ace their exams. Students must know that CBSE has reduced 30% syllabus this year and so they must carefully read the syllabus to appear for the 2021 exam.
In this article, we will discuss some important biology practical questions for class 12 students so that they can score good marks in their biology practical exam. However, before proceeding further, students must take a look at the practical exam pattern along with the marking scheme and the experiments expected to be carried out in the examination. This will give them an edge and help them to perform better in this subject.
Evaluation Scheme | Marks |
One Major Experiement 5,6 | 5 |
One Minor Experiement 2,3 | 4 |
Slide Preparation 1,4 | 5 |
Spotting | 7 |
Practical Record + Viva Voce (Credit to the student’s work over the academic session may be given) | 4 |
Investigatory project and its Project and its Record + Viva Voce (Credit to the student’s work over the academic session may be given) | 5 |
Total | 30 |
Part A: List of experiments
1. Prepare a temporary mount to observe pollen germination.
2. Collect and study soil from at least two different sites and study its texture, moisture content, pH and water holding capacity. Correlated with the kinds of plants found in them.
3. Collect water from two different water bodies around you and study them for pH, clarity and presence of any living organism.
4. Prepare a temporary onion root tip to study mitosis.
5. Study the effect of different temperatures or three different pH on the activity of salivary amylase on starch.
6. Isolate DNA from available plan material such as spinach, green pea seeds, papaya, etc.

Part B: Study/Observation of the following (Spotting)
1. Flowers adapted to pollination by different agencies (winds, insects and birds)
2. Identification of stages of gamete development i.e T.S of testis and T.S of ovaries through permanent slides (grasshopper/mice)
3. Meiosis in onion bud cell or grasshopper testis through permanent slides
4. T.S of blastula through permanent slides (Mammalian).
5. Prepared pedigree charts of any one of the genetic traits such as rolling of tongue, blood groups, ear lobes, widow’s peak and color blindness.
6. Common disease causing organism like Ascaris, Entamoeba, Plasmodium, any fungus causing ringworm through permanent slides, models, or virtual images. Comment on the synopsis of the disease that they cause.
7. Two plants and Two animals ( models/virtual images) found in xeric conditions. Comment upon their morphological adaptations.
8. Two plants and two animals (models/virtual images) found in aquatic conditions. Comment upon their morphological adaptations.
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Students must refer to the below-mentioned questions that can come in their practical examination. These questions are mostly asked by the examiners during the viva so students must prepare accordingly.
The above-mentioned questions are important for the biology practical exam. As we discussed above, students must be aware of the exam pattern, marking scheme and the entire practical list before starting their preparation so that they can easily attempt their paper. The practical exam comprises 30 marks and hence students must be fully prepared to ace this section along with their theory paper. Good luck!
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